Key result
Nascent mRNA forms a metastable hairpin-like structure during co-transcriptional folding instead of the G-quadruplex structure, sequestering its function as a ribosomal roadblock and allowing translation.
Why the study?
Intracellular mRNA folds co-transcriptionally during translation and can be entrapped in metastable structures, but the conformational dynamics disturbing G-quadruplex structures and their effects on translation remained unclear.
Population
Prokaryotic cells
Comparison
Metastable hairpin-like structure vs thermodynamically favored G-quadruplex structure
Authors
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Kinetic RNA folding may govern prokaryotic translation; leaves open extension to eukaryotic or cardiovascular gene regulation.
Kinetically formed RNA structures, such as metastable hairpin-like structures, have a dominant effect on gene expression in prokaryotes by sequestering G-quadruplex formation during co-transcriptional translation.
Endoh et al. (2019) studied this question. RNA G-rich sequence variants vs. Mutant sequences was evaluated on Protein expression levels (translation efficiency). Nascent mRNA forms a metastable hairpin-like structure during co-transcriptional folding instead of the G-quadruplex structure, sequestering its function as a ribosomal roadblock and allowing translation.
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