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Uridine diphospho-N-acetyl-d-mannosaminuronic acid and uridine diphospho-N-acetyl-d-glucosaminuronic acid were prepared chemically by the platinum-oxygen oxidation of uridine diphospho-N-acetyl-d-mannosamine and uridine diphospho-N-acetyl-d-glucosamine, respectively. In vitro incorporation of d-[14C]glucose from uridine diphospho-d-[14C]glucose into cell wall polysaccharide by the particulate enzyme fraction from Micrococcus lysodeikticus was dependent upon uridine diphospho-N-acetyl-d-mannosaminuronic acid. No polysaccharide was formed when uridine diphospho-N-acetyl-d-glucosaminuronic acid was supplied to the incorporation system in place of uridine diphospho-N-acetyl-d-mannosaminuronic acid. The particulate enzyme fraction also synthesized polysaccharide when supplied with the uridine diphospho-N-acetylhexosaminuronic acid preparation isolated from M. lysodeikticus cells. The isolated uridine diphospho-N-acetylhexosaminuronic acid preparations have been shown to be a mixture of two compounds, probably uridine diphospho-N-acetyl-d-mannosaaminuronic acid and uridine diphospho-N-acetyl-d-glucosaminuronic acid.
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Anderson et al. (1972) studied this question.
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