Deletion mapping of functional domains in the rotavirus capsid protein VP6.

View Full Paper

Ask AI

Bookmark

Population

Mammalian cells expressing mutant VP6 proteins of human rotavirus strain Wa

Comparison

Seven internal in-frame deletions within the… vs Wild-type VP6

Design

Preclinical

Authors

Discussion

Informs rotavirus VLP engineering; extends assembly domain mapping in animal models but leaves clinical translation open.

Population

Mammalian cells expressing mutant VP6 proteins of human rotavirus strain Wa

Intervention

Seven internal in-frame deletions within the coding region of gene 6

Comparator

Wild-type VP6

Outcome

Ability to oligomerize into trimers and assemble into double-layered virus-like particles upon coexpression with rotavirus core protein VP2surrogate

Identified specific amino acid domains in rotavirus VP6 critical for trimerization and assembly into virus-like particles.

Affranchino et al. (1997) studied this question.

View Full Paper

Ask AI

Bookmark