Key result
Real splice junction structures of eukaryotic and viral mRNA precursors were significantly more thermodynamically stable than random RNA sequences (0.354 vs 0.272 Kcals/nucleotide, p<0.05).
Absolute Event Rate: 0.354% vs 0.272%
p-value: p=<0.05
Thermodynamically stable secondary structures at splice junctions position exons favorably for ligation, suggesting a general mechanism for RNA splicing.
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May support stable RNA structures aiding exon ligation; hypothesis-generating for splicing mechanisms in eukaryotes and viruses.
Trapnell et al. (1980) studied this question. Real splice junction sequences vs. Random RNA sequences was evaluated on Mean specific stabilization energy (Kcals/nucleotide) (p=<0.05). Real splice junction structures of eukaryotic and viral mRNA precursors were significantly more thermodynamically stable than random RNA sequences (0.354 vs 0.272 Kcals/nucleotide, p<0.05).
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