Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices

Figures

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  Figure 1. Number of adipose ECM proteins classified by groups in human native AT (hAT) and human DAMs obtained by Method 1 (hDAM1) and Method 2 (hDAM2).

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  Figure 2. Number of adipose ECM proteins classified by groups in porcine native AT (pAT) and porcine DAMs obtained by Method 1 (pDAM1) and Method 2 (pDAM2).

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  Figure 3. Representative SEM images of the cellular morphology of RAW-264.7 macrophages after 24 h of culture on human (h) and porcine (p) DAMs obtained by the enzymatic method (hDAM1and pDAM1) and organic solvents treatment (hDAM2 and pDAM2). Characteristic filopodia are indicated by arrows.

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  Figure 4. Filopodia length of RAW-264.7 macrophages on human (h) and porcine (p) DAMs obtained by enzymatic method (hDAM1, pDAM1) and organic solvent treatment (hDAM2, pDAM2). The values, obtained after at least 150 C. albicans cells scored per experiment, are compared to control macrophages cultured on glass coverslips (horizontal line C). Statistical significance: ***: p < 0.005 (in comparison to the control); &: p < 0.05; &&&: p < 0.005 (hDAM1 vs. pDAM1, hDAM2 vs. pDAM2).

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  Figure 5. Representative confocal microscopy images of the Candida albicans/macrophage biointerface on the control surface (A,B) and human (h) and porcine (p) DAMs obtained by the enzymatic method and organic solvent treatment (C = hDAM1, D = hDAM2, E = pDAM1 and F = pDAM2). Nuclei were labeled in blue with DAPI, and the actin cytoskeleton was labeled in green with FITC-phalloidin. RFP-C. albicans (in red).

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  Figure 6. Chromatin condensation of RAW-264.7 macrophages cultured on the control surface (horizontal line C) and human (h) and porcine (p) DAMs obtained by the enzymatic method (hDAM1and pDAM1) and organic solvents treatment (hDAM2 and pDAM2) in presence of C. albicans (MOI 5). Inset: representative image of the chromatin condensation of RAW-264.7 macrophages on hDAM1 after C. albicans interaction. Statistical significance: *: p < 0.05; ***: p < 0.005 (comparison with respect to the control condition). ##: p < 0.01; ###: p < 0.005 (hDAM1 vs. hDAM2, pDAM1 vs. pDAM2). &&&: p < 0.005 (hDAM1 vs. pDAM1, hDAM2 vs. pDAM2).

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  Figure 7. TNFα (A) and IL-6 (B) production by RAW-264.7 macrophages cultured on control surface (horizontal line C) and human (h) and porcine (p) DAMs obtained by the enzymatic method (hDAM1and pDAM1) and organic solvents treatment (hDAM2 and pDAM2) in the presence or absence of C. albicans (MOI5). Statistical significance, *: p < 0.05; **: p < 0.01; ***: p < 0.005 (comparison with respect to the control condition). ###: p < 0.005 (hDAM1 vs. hDAM2, pDAM1 vs. pDAM2). &&&: p < 0.005 (hDAM1 vs. pDAM1, hDAM2 vs. pDAM2).