Using motility as the criterion of survival of spermatozoa, a method for the deep-frozen storage of rabbit semen was developed. The factors found to give optimum revival were the presence of 14% (v/v) dimethylsulphoxide during freezing, the inclusion of fructose in the diluent, and cooling to 5°C in an isotonic diluent while freezing in a hypertonic diluent.
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O’Shea et al. (1969) studied this question.
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