Reversible Ratiometric Fluorescent Probe for Sensing Bisulfate/H2O2 and Its Application in Zebrafish

Herein, a novel near-infrared fluorescent probe for ratiometric detection of bisulfate was designed and developed based on a conjugation of naphthopyran-benzothiazolium system. The sensor showed excellent selectivity, high sensitivity and a rapid response toward bisulfite in aqueous solution. Upon the addition of HSO 3 –, the sensor displayed 37-fold ( I 520 / I 630 ) fluorescence intensity enhancement, accompanied by an apparent color change from violet to colorless, suggesting that the sensor can be used to detect HSO 3 – with “naked-eye”. Notably, the addition product can be applied to the design of regenerative chemodosimeters based on the H 2 O 2 promoted elimination of bisulfite and recovery of probe 1 . Further cell and zebrafish imaging experiment demonstrated that the sensor could image the bisulfite/H 2 O 2 redox cycle in biological system with ratiometric manners.