Two important vectors of malaria in Africa, Anopheles gambiae and Anopheles arabiensis (Diptera: Culicidae), often occur sympatrically and cannot be distinguished morphologically. A chemical method was developed to identify individual laboratory-reared adult males or females of either species by extraction and analysis of cuticular components with gas chromatography. Statistically significant differences were seen between species when selected pairs of peaks were compared.
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Carlson et al. (1980) studied this question.
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