What question did this study set out to answer?

Determine the three-dimensional crystal structure of orotate phosphoribosyltransferase to understand its catalytic mechanism, substrate specificity, and structural relationship to other phosphoribosyltransferases.

February 15, 1994Biochemistry

Crystal Structure of Orotate Phosphoribosyltransferase

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Authors

GSGiovanna ScapinOxford Nanoimaging (United Kingdom)CGCharles GrubmeyerNatural Sciences and Engineering Research Council JSJames C. SacchettiniBoston University

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Implication

Structural analysis reveals the dimeric architecture of orotate phosphoribosyltransferase in Salmonella typhimurium, highlighting a conserved catalytic fold across nucleotide-synthesizing enzymes.

Key Points

Determine the three-dimensional crystal structure of orotate phosphoribosyltransferase to understand its catalytic mechanism, substrate specificity, and structural relationship to other phosphoribosyltransferases.
Solved the 2.6-Å resolution crystal structure of Salmonella typhimurium orotate phosphoribosyltransferase bound to orotidine monophosphate using multiple isomorphous replacement methods.
Constructed a structural model of human hypoxanthine-guanine phosphoribosyltransferase based on secondary structure predictions and the solved bacterial enzyme core.
Uncovered an enzyme subunit structure featuring a core five-stranded alpha/beta twisted sheet covered by an N-terminal region, creating a solvent-inaccessible pocket that dictates base specificity.
Identified that active orotate phosphoribosyltransferase forms a homodimer, with essential catalytic residues positioned across the inter-subunit interface.
Demonstrated that the alpha/beta core fold and phosphoribosyl pyrophosphate binding motif represent a conserved architectural scaffold shared across phosphoribosyltransferases.

Cite This Study

Scapin et al. (1994) studied this question.

synapsesocial.com/papers/6a9fa87392fe6ac2ccbab3cb https://doi.org/10.1021/bi00172a001

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Implication

Structural analysis reveals the dimeric architecture of orotate phosphoribosyltransferase in Salmonella typhimurium, highlighting a conserved catalytic fold across nucleotide-synthesizing enzymes.

Crystal Structure of Orotate Phosphoribosyltransferase

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Implication

Key Points

Cite This Study

Crystal Structure of Orotate Phosphoribosyltransferase

Discussion

Member takes

Implication

Key Points

Cite This Study

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