Glucose Production from Maltohexaose as a Substrate by the Bacterial Alpha‐amylase‐catalyzed Reaction. Its Time Courses Obtained by a Theoretical and an Experimental Approaches

Based on the subsite structure of an α‐amylase from Bacillus subtilis, production of glucose from a substrate maltohexaose in the amylase‐catalyzed reaction was theoretically predicted as a function of time. The theoretical time course was found consistent with the experimental results, which were obtained by the determination of a product glucose with a commercial assay‐kit. Hence, glucose, product from a substrate maltohexaose, will be a useful probe for the amylase‐catalyzed reaction.