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The described isocratic separation for phenylthiohydantoin (Pth) amino acid derivatives offers advantages over gradient systems: the baseline is more stable at high sensitivity, the resolution and reproducibility is excellent and the cost and consumption of solvent is very low. The separation of low picomolar quantities, on 2 different types of reverse phase columns, are presented. The method is used ‘off line’, i.e. as a separate analytical unit, or in an ‘on line’ mode, where it is directly connected to a protein sequencer for automatic identification of the released amino add derivatives.
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Ashman et al. (1985) studied this question.
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