Key result
A novel computer program identifies optimal regions for PCR amplification of highly variable genomes by deriving variability profiles and assessing DNA-primer hybrid stability.
The study presents a computational tool for designing PCR primers targeting highly variable genomes, demonstrated on foot-and-mouth disease virus.
May aid PCR primer design for variable viral genomes; leaves open validation before diagnostic adoption.
A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to identify optimal regions for PCR amplification, taking into account stability of DNA-primer hybrids. An application of the program to foot-and-mouth disease virus diagnosis is presented.
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Dopazo et al. (1993) studied this question. A novel computer program identifies optimal regions for PCR amplification of highly variable genomes by deriving variability profiles and assessing DNA-primer hybrid stability.
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