Application of Paper Chromatography to Avian Pathology

THERE is little or no information on the distribution of free amino acids among the tissues and in the body fluids of the fowl. Using filter paper chromatography, Ross et al. (1955) studied the free and bound amino acids in the blood serum and livers of healthy and Salm. pulloruminfected chickens. These workers demonstrated the presence of thirteen amino acids in the serum and sixteen in the livers of the uninfected chickens, but noted a reduction in the level of certain amino acids in the infected group. Since qualitative paper chromatography may prove to be of value in avian pathology, the study reported here was undertaken to determine before proceeding to pathological studies, the appearance of normal amino acid chromatograms of various healthy tissues of the fowl. EXPERIMENTAL Blood plasma, serum and tissues were obtained from Rhode Island Red males and females of different ages. Urine was obtained from ten …