Characterization of ovine granulosa cell-derived extracellular vesicles and their miRNA cargo following in vitro exposure to bisphenols A and S

Bisphenol A (BPA) and Bisphenol S (BPS) exposure disrupt ovarian function and granulosa cell (GC) steroidogenesis. Extracellular vesicles (EVs) and their miRNA cargo, as mediators of cellular response to environmental stimuli, might be involved in fertility and folliculogenesis. This study explored modulation of microRNA (miRNA) expression after 48 h BPA or BPS exposure (10 µM) in ovine primary GC and EVs from corresponding conditioned medium (CM EVs). Small RNA sequencing of control (0 h) and 48 h treated GC, CM EVs allowed identification of 526 ovine miRNAs, including 128 new sequences. BPA did not alter miRNA expression in GC, while BPS decreased cellular oar-24b miRNA. In contrast, BPA modified expression of 4 miRNAs in CM EVs, including 3 new sequences, and 2 miRNAs were modified by BPS. Both compounds reduced expression of a sequence homologous to miR-1306. Further studies are required to decipher their roles in bisphenol toxicity in GC.