Key result
Exposure of normal pleural mesothelial cells to TGF-β1 and IL-1β induced epithelial to mesenchymal transition, a phenotype also observed in cells isolated from tuberculous pleurisy effluents.
Pleural mesothelial cells may undergo epithelial to mesenchymal transition induced by TGF-β1 and IL-1β, contributing to pleural fibrosis in tuberculous pleurisy.
EMT may contribute to pleural fibrosis in tuberculous pleurisy; leaves open whether cytokine inhibition alters patient outcomes.
PURPOSE: Tuberculous pleurisy is the most frequent extrapulmonary manifestation of tuberculosis. In spite of adequate treatment, pleural fibrosis is a common complication, but the mechanism has not been elucidated. This study is to determine whether epithelial to mesenchymal transition (EMT) of mesothelial cells occurs in tuberculous pleurisy. MATERIALS AND METHODS: Normal pleural mesothelial cells, isolated from irrigation fluids during operations for primary spontaneous pneumothorax, were characterized by immunofluorescence and reverse transcription polymerase chain reaction (RT-PCR). These cells were treated in vitro with various cytokines, which were produced in the effluents of tuberculous pleurisy. The isolated cells from the effluents of tuberculous pleurisy were analyzed by immunofluorescence and RT-PCR analysis. RESULTS: The isolated cells from the irrigation fluid of primary spontaneous pneumothorax had epithelial characteristics. These cells, with transforming growth factor-β1 and/or interleukin-1β treatment, underwent phenotypic transition from epithelial to mesenchymal cells, with the loss of epithelial morphology and reduction in cytokeratin and E-cadherin expression. Effluent analysis from tuberculous pleurisy using immunofluorescence and RT-PCR demonstrated two phenotypes that showed mesenchymal characteristics and both epithelial & mesencymal characteristics. CONCLUSION: Our results suggest that pleural mesothelial cells in tuberculous pleurisy have been implicated in pleural fibrosis through EMT.
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Kim et al. (2010) studied Tuberculous pleurisy (n=12). TGF-β1 and IL-1β vs. Media only was evaluated on Morphologic and phenotypic changes indicative of epithelial to mesenchymal transition (EMT). Exposure of normal pleural mesothelial cells to TGF-β1 and IL-1β induced epithelial to mesenchymal transition, a phenotype also observed in cells isolated from tuberculous pleurisy effluents.
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