Although chromatography is a comparatively old procedure, the method of paper partition chromatography did not become popular until well after 1944 when Consden, Gordon and Martin introduced it and demonstrated its application. This tool was given renewed interest and greater utility as a result of the development of the ascending method of paper chromatography by Williams and Kirby in 1948. Since that time, chromatographic procedures with paper have been applied by a host of investigators to natural, synthetic, organic and inorganic substances. Bacteriologists, geneticists, botanists, zoologists and a variety of others have employed paper chromatography as an analytical method for the study of proteins, amino acids, carbohydrates, steroids, antibiotics, vitamins and many other substances.
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D. W. Micks (1956) studied this question.
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