Profiling of cell‐free DNA methylation and histone signatures in pediatric NAFLD: A pilot study

Figures

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  FIGURE 1. Global DNA methylation and histone signatures in plasma of pediatric patients with nonalcoholic fatty liver disease (NAFLD). (A) Percentage of cell‐free DNA (cfDNA) methylation in NAFLD (red) and control (green) patients. ***p < 0.001 vs. controls. (B) Box plots of the standardized individual histones expression in NAFLD (red) and controls (green); outliers were removed; the horizontal bars represent the medians and the whiskers. *p < 0.05 vs. controls. (C) Percentage of cfDNA methylation in patients with nonalcoholic steatohepatitis (NASH) NAFLD (red) and no NASH (yellow). ***p < 0.001 vs. controls. (D) Box plots of the standardized histones expression in patients with NASH (red) and no NASH (yellow); outliers were removed; the horizontal bars represent the medians and the whiskers.

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  FIGURE 2. Correlation between circulating cfDNA methylation and histological traits in patients with NAFLD. Correlation between percentages of cfDNA methylation in patients with NAFLD (n = 67) stratified by steatosis (A), lobular inflammation (B), fibrosis (C), ballooning (D), and NAFLD activity score (NAS) (E).

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  FIGURE 3. Correlation between circulating histones and histological traits in patients with NAFLD. Correlation between macroH2A1.2 histone expression and steatosis (A) or NAS (B) in patients with NAFLD (n = 60); macroH2A1.2 histone expressions were quantile‐normalized to produce these plots.

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  FIGURE 4. Performance of cfDNA methylation, patatin‐like phospholipase domain containing 3 (PNPLA3) rs738409, and macroH2A1.2 in predicting children with NASH. K‐nearest‐neighbors (KNN) receiver operating characteristic (ROC) curves achieved combining cfDNA methylation, PNPLA3 rs738409, and macroH2A1.2 features. True positive rate (TPR) and false positive rate (FPR) are indicated in the graph. Abbreviation: AUC, area under the curve.

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  FIGURE 5. Identification of the best model for predicting NASH. (A) Right bars represent the AUC achieved using the single feature values to predict the NASH status; red and green bars indicate, respectively, the positive and negative associations between the feature and the NASH status; left bars represent the log p value from the Mann–Whitney test for the same features performed on the NASH and no‐NASH NAFLD groups. (B) KNN ROC curves achieved by combining additional features to the cfDNA methylation and PNPLA3 rs738409 features. TPR and FPR are indicated in the graph.