Key result
L-arginine administration significantly reduced serum urea and creatinine levels, and attenuated glomerulosclerosis and interstitial fibrosis in 5-fluorouracil-treated rats.
Why the study?
Does L-arginine prevent 5-fluorouracil-induced nephrotoxicity in male albino rats?
Does L-arginine prevent 5-fluorouracil-induced nephrotoxicity in male albino rats?
Absolute Event Rate: 35.33% vs 85.13%
p-value: p=<0.05
L-arginine supplementation attenuates 5-fluorouracil-induced nephrotoxicity in rats by improving renal function and reducing histological damage and iNOS/caspase-3 expression.
L-arginine may attenuate 5-FU nephropathy in rats; hypothesis-generating and requires human trials before clinical consideration.
5-fluorouracil (5-FU) is a potent antineoplastic agent used for the treatment of various malignancies. The L-arginine nitric oxide (NO) pathway involved in the pathogenesis of chemotherapy induced kidney damage. This work investigated the beneficial mechanism of L-arginine supplementation in 5-FU induced nephropathy. Eighty male Wistar rats were divided into four equal groups: control group; L-arginine group (378 mg/rat/day for 4 weeks); 5-FU group (189 mg/rat/week for 4 weeks) and L-arginine for 1 week before and 4 weeks concomitant with 5-FU group. At the end of experiment, the kidney functions were assessed and kidneys specimens were processed for paraffin sections and stained with haematoxylin and eosin (H&E), Masson's trichome (MT) and periodic acid-Schiff (PAS) stains. Other sections were processed for immunohistochemical demonstration of caspase-3 and inducible NO synthase (iNOS). Image analyser was used to analyse the results morphometrically and statistically. L-arginine administration to 5-FU treated animals elicited significant reduction in serum urea and creatinine levels, urine volume, urinary protein excretion and kidney/body weight ratio in comparison to fluorouracil treated group. L-arginine improved glomeruloscelerosis, degeneration of convoluted tubules and interstitial fibrosis in 5-FU treated animals. L-arginine attenuated effectively some biochemical and histological changes in 5-FU nephrotoxicity.
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Badawoud et al. (2017) studied 5-fluorouracil-induced nephrotoxicity (n=80). L-arginine vs. 5-fluorouracil alone was evaluated on Serum urea level (mg/dL) (p=<0.05). L-arginine administration significantly reduced serum urea and creatinine levels, and attenuated glomerulosclerosis and interstitial fibrosis in 5-fluorouracil-treated rats.
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