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September 14, 2015Physiological ResearchOpen Access

Nanotherapeutics Shielded With a pH Responsive Polymeric Layer

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Key result

The transfection ability of the polymer-coated adenovirus at pH 7.4 was 75 times lower than at pH 5.4, demonstrating effective pH-responsive shielding and deshielding.

Population

Preclinical models including amino-modified latex nanoparticles and Adenovirus 5 encoding luciferase…

Comparison

Surface coating with pH-responsive HPMA-based… vs Uncoated vectors or coated vectors at…

Design

Preclinical

Authors

LKLibor KostkaVŠVladimír ŠubrRLRichard Laga

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Overview

May advance nanotherapeutic gene delivery for cardiovascular targets; hypothesis-generating and requires in vivo validation before clinical consideration.

Structured PICO

P
Population
Preclinical models including amino-modified latex nanoparticles and Adenovirus 5 encoding luciferase reporter gene tested in prostate cancer PC3 cells
I
Intervention
Surface coating with pH-responsive HPMA-based copolymers (e.g., poly(HPMA-co-Ma-Ah-NHN=OHA-TT))
C
Comparator
Uncoated vectors or coated vectors at physiological pH (7.4)
O
Outcome
Transfection ability (luciferase expression) and hydrodynamic radius (Rh)

A novel pH-responsive HPMA-based copolymer coating for adenoviral vectors successfully shields the vector at physiological pH and restores transfection ability in acidic environments.

Cite This Study

Kostka et al. (2015) studied this question. pH-responsive HPMA-based copolymers vs. Uncoated virus or different pH was evaluated on Transfection ability. The transfection ability of the polymer-coated adenovirus at pH 7.4 was 75 times lower than at pH 5.4, demonstrating effective pH-responsive shielding and deshielding.

synapsesocial.com/papers/6a9fcf1786dc1a12ec5cc426https://doi.org/10.33549/physiolres.933139
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  3. 3PEGylation of Adenovirus with Retention of Infectivity and Protection from Neutralizing Antibody in Vitro and in Vivo1999 · 410 citations