Bypassing the Bottleneck: Broad-host geminivirus enables systemic, tissue-culture-free plant genome editing

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A broad-host geminivirus enables systemic plant genome editing without the need for tissue culture. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Ved Prakash; Praveen Khatri

doi:10.1093/plphys/kiag652

September 5, 2026PLANT PHYSIOLOGYOpen Access

Bypassing the Bottleneck: Broad-host geminivirus enables systemic, tissue-culture-free plant genome editing

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Key Points

To develop a systemic, tissue-culture-free plant genome editing platform using a broad-host geminivirus vector.
Engineered a broad-host-range geminivirus vector capable of delivering genome editing reagents systemically through plant vascular systems.
Delivered viral constructs directly into host plants to achieve mutagenesis without requiring in vitro tissue culture or plant regeneration steps.
Enabled systemic movement and expression of genome editing machinery across diverse plant tissues.
Successfully bypassed traditional tissue culture bottlenecks to achieve targeted plant genome modifications across a broad host range.

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Implication

Laboratory study demonstrates systemic, tissue-culture-free genome editing in plants using a broad-host geminivirus, indicating a streamlined route to overcome regeneration bottlenecks.

Abstract

Ved Prakash, Praveen Khatri; Bypassing the Bottleneck: Broad-host geminivirus enables systemic, tissue-culture-free plant genome editing, Plant Physiology,

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Cite This Study

Prakash et al. (2026) studied this question.

synapsesocial.com/papers/6a9fd72a58e84d0ff5b45b48 https://doi.org/10.1093/plphys/kiag652

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Bypassing the Bottleneck: Broad-host geminivirus enables systemic, tissue-culture-free plant genome editing

Key Points

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Implication

Abstract

Discussion

Cite This Study

Bypassing the Bottleneck: Broad-host geminivirus enables systemic, tissue-culture-free plant genome editing

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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