What does this research mean for the field?

Organic acids from Lonicera japonica Thunb., particularly caffeic acid methyl ester (CAME), exhibit anti-colorectal cancer effects by inhibiting cell proliferation and migration and inducing apoptosis, potentially through the downregulation of ERK phosphorylation. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To investigate the therapeutic potential, molecular targets, and underlying anticancer mechanisms of organic acids derived from Lonicera japonica Thunb. against colorectal cancer.

September 8, 2026Discover OncologyOpen Access

Network pharmacology, multi-omics analysis, and preliminary in vitro validation reveal the anti-colorectal cancer potential of Lonicera japonica Thunb. organic acids

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Authors

CHChen HuiyingShanxi Medical University XNXiyun NiShanxi Medical University LLLi LiShanxi Medical University

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Implication

In vitro study demonstrates reduced proliferation and ERK signaling in colorectal cancer cells treated with Lonicera japonica acids, highlighting candidate natural therapeutics.

Key Points

To investigate the therapeutic potential, molecular targets, and underlying anticancer mechanisms of organic acids derived from Lonicera japonica Thunb. against colorectal cancer.
Screened active organic acids and mapped potential targets using network pharmacology, random forest algorithms, molecular docking, and transcriptomic data from GEPIA, TCGA, and GEO.
Evaluated the anticancer activity of caffeic acid methyl ester (CAME) in human colorectal cancer HCT116 cells using CCK-8, colony formation, wound healing, Transwell, and flow cytometry assays.
Measured total ERK and phosphorylated ERK (p-ERK) protein levels via Western blot following treatment with CAME alone or combined with the ERK pathway inhibitor SCH772984.
Identified four representative organic acids and 117 potential targets enriched in immune, inflammatory, and oncogenic signaling pathways, prioritizing DPP4 and MAPK1 as core targets.
CAME significantly inhibited HCT116 cell proliferation in a dose- and time-dependent manner (IC50 = 16.06 µM at 48 h), impaired colony formation, suppressed cell migration, and induced apoptosis.
CAME treatment significantly downregulated p-ERK protein levels, and co-treatment with SCH772984 further reduced HCT116 cell viability and p-ERK expression.

Cite This Study

Huiying et al. (2026) studied this question.

synapsesocial.com/papers/6a9fd7e758e84d0ff5b470ca https://doi.org/10.1007/s12672-026-05849-6

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Implication

In vitro study demonstrates reduced proliferation and ERK signaling in colorectal cancer cells treated with Lonicera japonica acids, highlighting candidate natural therapeutics.

Network pharmacology, multi-omics analysis, and preliminary in vitro validation reveal the anti-colorectal cancer potential of Lonicera japonica Thunb. organic acids

Discussion

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Implication

Key Points

Cite This Study

Network pharmacology, multi-omics analysis, and preliminary in vitro validation reveal the anti-colorectal cancer potential of Lonicera japonica Thunb. organic acids

Discussion

Member takes

Implication

Key Points

Cite This Study

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