Spectral Imaging and Autonomous Inspection Technologies for Nutrient Diagnosis of Protected Horticultural Crops: A Review

Protected horticultural crops are commonly produced at high planting densities and have short production cycles; imbalances in water and fertilizer supply can rapidly affect plant vigor, yield, and quality. Non-destructive diagnostic methods are therefore needed to characterize plant nutritional status under greenhouse conditions. Spectral imaging can simultaneously capture spatial and spectral information associated with pigments, water status, tissue structure, and canopy phenotype. It does not directly detect nutrient ions; rather, it captures physiological and structural responses that may be associated with nutrient status and may also be influenced by water deficit, disease, temperature, salinity, phenology, and genotype. This review focuses on crops grown in soil, substrate, and hydroponic systems under greenhouse conditions. Studies conducted in vertical farms, growth chambers, and open fields are included only as supplementary references for sensor selection, model calibration, and inspection methods. This article synthesizes diagnostic indicators for nitrogen, phosphorus, and potassium, together with their associated physiological responses and spectral characteristics; compares the performance of hyperspectral, multispectral, and machine learning methods at the leaf, plant, and canopy scales; and examines fixed measurement, stop-and-go mobile inspection, continuous motion imaging, and autonomous plant revisitation. Existing studies have established a solid foundation for nutrient content retrieval, deficiency identification, and mobile monitoring. However, several challenges remain inadequately addressed under continuous inspection conditions, including radiometric–geometric joint calibration, plant identity preservation, acquisition of multi-element chemical truth values, model generalization across growth stages and greenhouse types, and long-term performance evaluation. Future work should refine standardized protocols for dynamic data collection and water–fertilizer environmental control, integrate mechanistic constraints with data driven approaches, and incorporate plant re-identification, spatiotemporal registration, uncertainty quantification, and online calibration. These efforts will contribute to constructing a long-term stable and comparable nutritional diagnostic system, thereby advancing the transition of facility vegetable nutritional monitoring from single-time static measurements toward continuous, traceable, and autonomously patrolled systems that may ultimately support precision irrigation and fertilization management after appropriate independent validation.