January 1, 2002Journal of Vascular Research

Real-Time Detection of Activation Patterns in Individual Platelets during Thromboembolism in vivo: Differences between Thrombus Growth and Embolus Formation

Q: What is the clinical evidence from this study?

Study design: Other. Population: Thromboembolism. Intervention: Real-time detection of activation patterns using fluo-3 labeled platelets vs. Thrombus growth vs embolus formation. Primary outcome: Intracellular free Ca(2+) concentration ([Ca(2+)](i)) and platelet activation patterns.

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Key result

Platelets adhering to a stationary thrombus exhibited a prolonged intracellular calcium increase and degranulation, whereas those adhering to a growing embolus showed only a transient calcium rise.

Population

Anesthetized rabbits (preclinical model)

Design

Preclinical

Follow-up

30 minutes after injection

Authors

MGMiriam A. van Gestel JHJohan W. M. Heemskerk DSDick W. Slaaf

Discussion

Member takes

Overview

Highlights distinct platelet calcium signaling in stable versus embolizing thrombi in vivo; leaves open agonist-specific targeting to modulate embolization risk.

Structured PICO

Population

Anesthetized rabbits injected with fluo-3 labeled platelets to study thromboembolism in vivo.

Intervention

Injection of isolated platelets labeled with Ca(2+)-sensitive fluorescent probe fluo-3, followed by wall puncture of mesenteric arterioles to induce thrombus formation

Outcome

Real-time detection and quantification of activation of individual platelets using intracellular free Ca(2+) concentration ([Ca(2+)](i))surrogate

Single platelet activation patterns differ significantly between thrombus growth and embolus formation in vivo, suggesting different agonist mechanisms are involved in each process.

Cite This Study

Gestel et al. (2002) studied Thromboembolism. Real-time detection of activation patterns using fluo-3 labeled platelets vs. Thrombus growth vs embolus formation was evaluated on Intracellular free Ca(2+) concentration ([Ca(2+)](i)) and platelet activation patterns. Platelets adhering to a stationary thrombus exhibited a prolonged intracellular calcium increase and degranulation, whereas those adhering to a growing embolus showed only a transient calcium rise.

synapsesocial.com/papers/6a9fdd0afeb70227d67c66bb https://doi.org/10.1159/000067208

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January 1, 2002Journal of Vascular Research

Real-Time Detection of Activation Patterns in Individual Platelets during Thromboembolism in vivo: Differences between Thrombus Growth and Embolus Formation

View Full Paper

Ask AI

Bookmark

Key result

Platelets adhering to a stationary thrombus exhibited a prolonged intracellular calcium increase and degranulation, whereas those adhering to a growing embolus showed only a transient calcium rise.

Population

Anesthetized rabbits (preclinical model)

Design

Preclinical

Follow-up

30 minutes after injection

Authors

MGMiriam A. van Gestel JHJohan W. M. Heemskerk DSDick W. Slaaf

Discussion

Member takes

Overview

Highlights distinct platelet calcium signaling in stable versus embolizing thrombi in vivo; leaves open agonist-specific targeting to modulate embolization risk.

Structured PICO

Population

Anesthetized rabbits injected with fluo-3 labeled platelets to study thromboembolism in vivo.

Intervention

Injection of isolated platelets labeled with Ca(2+)-sensitive fluorescent probe fluo-3, followed by wall puncture of mesenteric arterioles to induce thrombus formation

Outcome

Real-time detection and quantification of activation of individual platelets using intracellular free Ca(2+) concentration ([Ca(2+)](i))surrogate

Single platelet activation patterns differ significantly between thrombus growth and embolus formation in vivo, suggesting different agonist mechanisms are involved in each process.

Cite This Study

synapsesocial.com/papers/6a9fdd0afeb70227d67c66bb https://doi.org/10.1159/000067208

View Full Paper

Ask AI

Bookmark