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June 1, 1964The Journal of Immunology

Radioimmunoelectrophoresis of Thyroid Antigens and Anti-Thyroglobulin Antibodies in Clinical and Experimental Thyroiditis

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Authors

HGHoward C. GoodmanUniversity of Southern CaliforniaEEE. D. ExumNational Institutes of HealthJRJacob RobbinsMichigan State University

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Overview

Laboratory investigation reveals multiple antibody classes targeting heterogeneous thyroglobulin in human and guinea pig thyroiditis, suggesting diverse autoimmune responses across species.

Key Points

  • To analyze thyroid antigens and characterize anti-thyroglobulin antibody classes in human and guinea pig thyroiditis using radioimmunoelectrophoresis.
  • Coupled immunoelectrophoresis with autoradiography to track radiolabeled thyroid antigens and corresponding serum antibodies.
  • Used a double precipitation technique reacting labeled antigens with antibodies previously precipitated by anti-serum proteins in human and guinea pig samples.
  • Radioactive precipitin lines between thyroglobulin and anti-thyroglobulin demonstrated immunochemical heterogeneity among thyroglobulin molecules.
  • Iodoalbumin was identified in thyroid extracts of both species, while iodo-γ-globulin was detected specifically in a patient with chronic lymphocytic thyroiditis.
  • Human sera contained three anti-thyroglobulin antibody classes (slow γ-globulin, fast γ-globulin, and macroglobulin), whereas guinea pig macroglobulin lacked anti-thyroglobulin activity.

Cite This Study

Goodman et al. (1964) studied this question.

synapsesocial.com/papers/6a9fdf9e8b5dbbb92fe61c8ehttps://doi.org/10.4049/jimmunol.92.6.843
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