Functional Sites of Transfer RNA for the Binding to Messenger RNA-Ribosome Complex

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Structured PICO

Population

In vitro model studying tRNA molecules and mRNA-ribosome complexes

Intervention

Purified tetranucleotide TpψpCpGp, deaminated tRNA, and tRNA digested by phosphodiesterases

Comparator

Terminal-pCpCpA-free tRNA fragments, terminal adenosine-oxidized tRNA, and non-aminoacylated tRNA

Outcome

Inhibitory effects on aminoacyl-tRNA synthesis and aminoacyl-tRNA binding to mRNA-ribosome complexsurrogate

The sequence TpψpCpGp in tRNA is required for aminoacyl-tRNA binding to the mRNA ribosome complex, but not for aminoacyl-tRNA synthesis.

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Does not support clinical translation; hypothesis-generating for tRNA sequence function in ribosomal binding.

Shimizu et al. (1970) studied this question.

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