High‐Resolution Characterization of Intrinsic Disorder in Proteins: Expanding the Suite of 13C‐Detected NMR Spectroscopy Experiments to Determine Key Observables

Order in disorder: The characterization of intrinsically disordered proteins by NMR spectroscopy is a necessity on the one hand and a continuous challenge on the other. We propose two experiments that provide diagnostic parameters to monitor the degree of unfolding of a polypeptide. The test was performed on the yeast Cox17 protein, known to gain its function through maturation from an intrinsically disordered state (see figure).