Key result
The La motif contributes to RNA binding specificity, RRM1 is indispensable for association with pre-tRNA, and RRM2 is needed for full-affinity binding in vitro but not crucial in vivo.
Population
human La autoantigen (hLa) protein in vitro and in vivo models
Design
Preclinical
Authors
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No clinical implications for La-related disorders; extends mechanistic mapping of RNA-binding domains for future studies.
The RNA binding domains of the human La autoantigen contribute individually and sequentially to RNA recognition and subcellular localization.
Horke et al. (2004) studied this question. RNA binding domains of human La autoantigen (La motif, RRM1, RRM2) was evaluated on RNA binding affinity, specificity, and subcellular localization. The La motif contributes to RNA binding specificity, RRM1 is indispensable for association with pre-tRNA, and RRM2 is needed for full-affinity binding in vitro but not crucial in vivo.
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