Protective Effects of Cuscutae Semen Extract from Oxidative Stress and Amyloid β25-35-Induced SH-SY5Y Neuronal Cell Dysfunction

Objectives: Amyloid beta (Aβ) is generated by the amyloidogenic pathway.Its accumulation is a major cause of Alzheimer's disease.The present study investigates the free radical scavenging activities and inhibition of Aβ 25-35 -induced neurotoxicity by Cuscutae semen (CS) water extract treatment.Methods: The scavenging activities of radicals, including 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH), and nitric oxide (NO) of the CS water extract were assessed.Moreover, the SH-SY5Y cells were used to investigate cell viability, inflammation-related protein expression, and inflammatory cytokine secretion using CS water extract to determine neuroprotective effects.Results: The CS water extract inhibited the production of free radicals such as DPPH, OH, and NO, dose-dependently.Meanwhile, oxidative stress was induced, and cell viability was decreased by Aβ 25-35 .The CS water extract treatment significantly increased cell viability in Aβ 25-35 -stimulated SH-SY5Y cells.Additionally, the treatment with CS water extract significantly down-regulated the expression of inducible nitric oxide synthase and cyclooxygenase-2 in Aβ 25-35 -stimulated SH-SY5Y cells.Secretion of interleukin-1β and interleukin-6 in Aβ 25-35 -induced SH-SY5Y cells was significantly inhibited by CS water extract.Conclusions: This study indicated the free radical scavenging activities and neuroprotective effects of CS water extract.