A procedure is described which allows one to deduce from flash photolysis data (absorbance change vs time) recorded at different temperatures the natural rate constants for the elementary reaction steps and the transient absorption spectra of the intermediates within a given kinetic scheme. The selected solutions fulfil two requirements: (i) the rate constants for different temperatures follow Arrhenius’law; (ii) the absorption spectra of the intermediates are independent of temperature. The method is applied to the photocycle of bacteriorhodopsin; the selected model comprises two L‐species, a branching at the M‐intermediate directly back to BR and an equilibrium between M and O.
No takes yet. Share an insight, caveat, or question.
Maurer et al. (1987) studied this question.
Synapse has enriched 5 related papers. Consider them for comparative context: