Summary Seven independent transformants were previously shown to carry T‐DNA inserts that are genetically linked to the self‐incompatibility ( S )‐locus in Petunia hybrida . These T‐DNAs provided reliable targets for fluorescent in situ hybridisation (FISH) localisation. The T‐DNA loci were found to be distributed over the entire long arm of chromosome III. The most tightly linked T‐DNA loci were located in a subcentromeric position. Independent confirmation of this assignment of the S ‐locus to chromosome III was obtained by RFLP analysis of a cDNA marker linked to the S ‐locus of potato (CP100). Tight linkage was found between CP100 and a peroxidase isozyme locus previously mapped to chromosome III. The co‐localisation of CP100, peroxidase and the S ‐locus in petunia reveals synteny around the S ‐locus between four members of the Solanaceae family. The implications of a subcentromeric localisation of the S ‐locus are discussed in relation to chromosome fragments associated with self‐compatible mutants.
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Hoopen et al. (1998) studied this question.