Students in the biophysical chemistry laboratory use 1 H NMR to evaluate the equilibrium constant for the cis-trans isomerism of N- acetyl-L-proline. The four solvent systems (45:55 d 6 -benzene-CDCl 3, d 6 -acetone, D 2 O with pH < 2, and D 2 O with pH > 7) demonstrate the effects of solvent polarity and aqueous pH.
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Williams et al. (2002) studied this question.
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