What question did this study set out to answer?

Identify and characterize the cryptic diterpene synthase responsible for talaronoid biosynthesis in the marine-derived fungus Aspergillus flavipes.

September 20, 2022Organic LettersOpen Access

Identification and Characterization of a Cryptic Bifunctional Type I Diterpene Synthase Involved in Talaronoid Biosynthesis from a Marine-Derived Fungus

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Authors

PZPeng Zhang GWGuangwei Wu SHStephanie C. Heard

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Overview

Experimental study reveals the biosynthetic pathway of talaronoid C in Aspergillus flavipes, highlighting the role of a cryptic bifunctional diterpene synthase.

Key Points

Identify and characterize the cryptic diterpene synthase responsible for talaronoid biosynthesis in the marine-derived fungus Aspergillus flavipes.
Identified the tnd biosynthetic gene cluster from Aspergillus flavipes and performed in vivo heterologous expression of a cryptic bifunctional type I diterpene synthase.
Conducted 13C-labeling and stable isotope tracer experiments to track the enzymatic cyclization of geranylgeranyl diphosphate into downstream metabolites.
Heterologous expression of the diterpene synthase generated talarodiene, a novel diterpene backbone featuring a benzo[a]cyclopenta[d]cyclooctane tricyclic fused ring system.
Isotopic labeling demonstrated that geranylgeranyl diphosphate converts directly into talarodiene, which undergoes biotransformation to form talaronoid C.

Cite This Study

Zhang et al. (2022) studied this question.

synapsesocial.com/papers/6a9ffe96445f3771f4e620fe https://doi.org/10.1021/acs.orglett.2c02904

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September 20, 2022Organic LettersOpen Access

Identification and Characterization of a Cryptic Bifunctional Type I Diterpene Synthase Involved in Talaronoid Biosynthesis from a Marine-Derived Fungus

View Full Paper

Ask AI

Bookmark

Authors

PZPeng Zhang GWGuangwei Wu SHStephanie C. Heard

Discussion

Member takes

Overview

Experimental study reveals the biosynthetic pathway of talaronoid C in Aspergillus flavipes, highlighting the role of a cryptic bifunctional diterpene synthase.

Key Points

Identify and characterize the cryptic diterpene synthase responsible for talaronoid biosynthesis in the marine-derived fungus Aspergillus flavipes.
Identified the tnd biosynthetic gene cluster from Aspergillus flavipes and performed in vivo heterologous expression of a cryptic bifunctional type I diterpene synthase.
Conducted 13C-labeling and stable isotope tracer experiments to track the enzymatic cyclization of geranylgeranyl diphosphate into downstream metabolites.
Heterologous expression of the diterpene synthase generated talarodiene, a novel diterpene backbone featuring a benzo[a]cyclopenta[d]cyclooctane tricyclic fused ring system.
Isotopic labeling demonstrated that geranylgeranyl diphosphate converts directly into talarodiene, which undergoes biotransformation to form talaronoid C.

Cite This Study

Zhang et al. (2022) studied this question.

synapsesocial.com/papers/6a9ffe96445f3771f4e620fe https://doi.org/10.1021/acs.orglett.2c02904

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