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Water samples were collected from a soft water highly humic pond, and all particulates of >0.15‐μm nominal diameter were removed by continuous flow centrifugation. Freezing caused the formation of brown particulates which could be removed by centrifugation. The particulates can be dispersed by sonification, but the size distribution of organic fractions is permanently altered by freezing.
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Giesy et al. (1978) studied this question.
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