Key result
Defects in 23S rRNA processing resulted in an accumulation of ribosomal subunits and caused a significant delay in ribosome assembly in Escherichia coli.
Precursor residues in 23S rRNA control ribosome assembly in E. coli, suggesting a regulatory role coupling assembly to rRNA processing.
In Escherichia coli, rRNAs are initially transcribed with precursor sequences, which are subsequently removed through processing reactions. To investigate the role of precursor sequences, we analyzed ribosome assembly in strains containing mutations in the processing RNases. We observed that defects in 23S rRNA processing resulted in an accumulation of ribosomal subunits and caused a significant delay in ribosome assembly. These observations suggest that precursor residues in 23S rRNA control ribosome assembly and could be serving a regulatory role to couple ribosome assembly to rRNA processing. The possible mechanisms through which rRNA processing and ribosome assembly could be linked are discussed.
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Gutgsell et al. (2012) studied Escherichia coli ribosome assembly. Mutations in processing RNases was evaluated on Ribosome assembly. Defects in 23S rRNA processing resulted in an accumulation of ribosomal subunits and caused a significant delay in ribosome assembly in Escherichia coli.
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