Key result
A mouse muscle cDNA encoding the basic isozyme of adenylosuccinate synthetase was cloned and expressed, yielding a functional 50 kDa protein with high expression in skeletal muscle and heart tissue.
Population
Mouse tissues, purified rat muscle enzyme, and COS cells
Design
Preclinical
Authors
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Enables genetic dissection of adenylosuccinate synthetase in muscle; leaves open any role in human cardiac or skeletal disease.
The cloning of the mouse muscle cDNA for adenylosuccinate synthetase provides tools for future genetic and biochemical analysis of its role in muscle physiology.
Guicherit et al. (1991) studied this question. Molecular cloning and expression of mouse muscle cDNA was evaluated on Cloning and expression of adenylosuccinate synthetase. A mouse muscle cDNA encoding the basic isozyme of adenylosuccinate synthetase was cloned and expressed, yielding a functional 50 kDa protein with high expression in skeletal muscle and heart tissue.
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