High‐performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light‐scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soybeans subjected to storage at high moisture content. Analytical and preparative separations of phospholipids by normal‐phase HPLC were accomplished. Major class fractions were analyzed by transmethylation and capillary collumn chromatography for fatty acid composition, and by reverse‐phase C‐18 HPLC (RP‐HPLC) for molecular species composition. The RP‐HPLC‐ELSD system was limited to the analysis of phosphatidylcholine and phosphatidylethanolamine.
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Mounts et al. (1992) studied this question.
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