Key result
A 6-minute ventricular fibrillation cardiac arrest followed by 2 minutes of basic life support and adrenaline resulted in 88% survival at 14 days compared to 67% for an 8-minute arrest, with both durations causing graded neurologic and histopathologic damage.
Absolute Event Rate: 88% vs 67%
This study establishes a dynamic global ischemia rodent model of ventricular fibrillation cardiac arrest that produces graded neurologic and histopathologic damage, suitable for testing neuroprotective therapies.
Shorter VF arrest may improve survival; leaves open optimal resuscitation timing and neuroprotection targets in humans.
PURPOSE: The aim of the study was to establish a ventricular fibrillation (VF) cardiac arrest (CA) resuscitation model with consistent neurologic and neuropathologic damage as potential therapeutic target. METHODS: Prospectively randomized groups of experiments in two phases. In phase 1 four groups of male Sprague-Dawley rats (n = 5) were resuscitated after 6 min VFCA with 2 and 6 min basic life support durations (BLS) with and without adrenaline. In phase 2 the most promising group regarding return of spontaneous circulation (ROSC) and survival was compared with a group of 8 min CA. Resuscitability, neurologic deficit scores (NDS), and overall performance category (OPC) were assessed daily; histolopathology of the hippocampal CA1 region [hematoxylin and eosin- (viable neurons), Fluoro-Jade- (dying neurons), and Iba-1 immunostaining (microglial activation-semiquantitative)] on day 14. RESULTS: Two minutes BLS and with adrenaline as most promising group of phase 1 compared with an 8 min group in phase 2 exhibited ROSC in 8 (80%) vs. 9 (82%) animals and survivors till day 14 in 7 (88%) (all OPC 1, NDS 0 ± 0) vs. 6 (67%) (5 OPC 1, 1 OPC 2, NDS 0.83 ± 2.4) animals. OPC and NDS were only significantly different at day 1 (OPC: P = 0.035; NDS: P = 0.003). Histopathologic results between groups were not significantly different; however, a smaller variance of extent of lesions was found in the 8 min group. Both CA durations caused graded neurologic, overall, such as histopathologic damage. CONCLUSIONS: This dynamic global ischemia model offers the possibility to evaluate further cognitive and novel neuroprotective therapy testing after CA.
No takes yet. Share an insight, caveat, or question.
Ettl et al. (2017) studied Ventricular fibrillation cardiac arrest (n=49). 6-minute cardiac arrest with 2 minutes basic life support and adrenaline vs. 8-minute cardiac arrest with 2 minutes basic life support and adrenaline was evaluated on Survival till day 14 among animals with return of spontaneous circulation. A 6-minute ventricular fibrillation cardiac arrest followed by 2 minutes of basic life support and adrenaline resulted in 88% survival at 14 days compared to 67% for an 8-minute arrest, with both durations causing graded neurologic and histopathologic damage.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: