Spores suspended in water were heated at 73·5, 100, 115 and 121° and colony counts made. These showed an initial increase followed by an exponential decline. Spores in phosphate buffer showed a reduced activation. Comparison of total (microscopic) and colony counts showed that only about 5% of unheated spores developed into colonies. This increased to about 50% after optimum heat activation.
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Cook et al. (1964) studied this question.
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