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A rapid, sensitive, reverse-phase high pressure liquid chromatographic assay was developed for the simultaneous determination of naproxen and salicylic acid. These compounds are extracted from serum and then separated on a reverse-phase column using an acidified methanol eluent. Utilization of a flow-through fluorescence detector in series with a variable wavelength micro-UV detector enhances the sensitivity of the assay. Application of the assay to therapeutic levels of the drugs in human serum is demonstrated.
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Burgoyne et al. (1980) studied this question.
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