Key result
In skeletal muscle fibers, the regulatory head of troponin acts as a pure Ca2+ sensor tilting by ~30° upon activation, whereas the IT arm is primarily sensitive to myosin head binding.
Population
Demembranated skeletal muscle fibers from adult New Zealand White rabbits
Comparison
Bifunctional rhodamine probes on the C and E… vs Relaxing conditions (pCa 9) or native TnC
Design
Preclinical
Authors
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Hypothesis-generating for thin-filament regulation in skeletal muscle; leaves open translation to human cardiac troponin function.
In situ, the regulatory head of troponin acts as a pure Ca2+ sensor, whereas the IT arm is primarily sensitive to myosin head binding.
Sun et al. (2006) studied Skeletal muscle activation (basic science). Calcium and myosin binding vs. Relaxed muscle (pCa 9) was evaluated on Orientation of troponin C helices (C and E) measured by polarized fluorescence. In skeletal muscle fibers, the regulatory head of troponin acts as a pure Ca2+ sensor tilting by ~30° upon activation, whereas the IT arm is primarily sensitive to myosin head binding.
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