Key result
The morphological grade of erythrocytes gradually decreased over time, with significant differences based on the type of anticoagulant and animal species used.
Why the study?
A method for evaluating the specific type of anticoagulant and blood characteristics of each animal species was needed to ensure consistent and reliable results in in vitro blood circulation loop systems.
Does the type of anticoagulant and animal species affect erythrocyte morphology in an in vitro blood circulation loop system?
Does the type of anticoagulant and animal species affect erythrocyte morphology in an in vitro blood circulation loop system?
The hemocompatibility of in vitro blood circulation loop systems varies depending on the animal species and anticoagulant used, highlighting the need for standardized control measures like observing erythrocyte morphology.
Species-specific anticoagulant validation may enhance in vitro hemocompatibility reliability; leaves open standardized protocols across animal blood models.
Background: In vitro blood circulation loop systems are utilized to assess the hemocompatibility and performance of medical devices that come into contact with blood, in accordance with the international standards ASTM F1830 and ASTM F1841. However, a method for evaluating the specific type of anticoagulant and the blood characteristics of each animal species is necessary to ensure consistent and reliable results. Methods: Blood was collected from healthy rabbits, pigs, rhesus monkeys, and cynomolgus monkeys to evaluate whole blood preserved in anticoagulants (ACD-A, CPDA-1, and heparin). For each sample, red blood cells were monitored over time, and their morphological characteristics were documented. Results: The morphological grade of erythrocytes gradually decreased over time. Significant differences were observed based on the type of anticoagulant used in the experiment, and variations were noted among different animal species. Conclusions: The hemocompatibility of in vitro blood circulation loop systems may vary depending on the animal species. Observing erythrocyte morphology can serve as a control measure to ensure reproducible results.
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Kim et al. (2025) studied this question. Anticoagulants (ACD-A, CPDA-1, and heparin) was evaluated on Morphological grade of erythrocytes over time. The morphological grade of erythrocytes gradually decreased over time, with significant differences based on the type of anticoagulant and animal species used.
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