Key result
The addition of sodium azide to the enzyme-conjugated solution, adsorption of anti-human embryo lung antibodies, and coating wells with 1% BSA effectively eliminated nonspecific reactions in ELISA for varicella, measles, and rubella viruses.
Why the study?
Does the addition of sodium azide, anti-HEL antibody adsorption, and BSA coating eliminate nonspecific reactions in micro-ELISA for viral antibodies?
Does the addition of sodium azide, anti-HEL antibody adsorption, and BSA coating eliminate nonspecific reactions in micro-ELISA for viral antibodies?
Specific modifications to the micro-ELISA protocol successfully eliminate nonspecific reactions, allowing for accurate routine serological testing of varicella zoster, measles, and rubella viruses.
These modifications may enhance ELISA specificity for viral serology; leaves open clinical validation before routine diagnostic use.
The enzyme-linked immunosorbent assay (ELISA) was developed by Engvall & Perlmann. Recently Voller & Bidwell introduced a micro modification of this assay for detection of viral antibodies.It is known that many nonspecific factors may affect the outcome of this test. These factors must be eliminated for application to the routine serological test.In this study micro-ELISA is adapted for varicella zoster, measles and rubella virus by elimination of nonspecific reaction. The procedures as follows enable us to detect each antibody.1. The addition of sodium azide to the enzyme-conjugated solution and the adsorption anti -human embryo lung antibodies from sera employed are effective factors in the detection of ELISA antib odies to varicella zoster virus.2. And in the detection of antibodies to measles and rubella virus, the addition of sodium azids and coating sensitized wells with I% BSA are effective in the success of the ELISA procedure.
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Mizutani et al. (1981) studied Varicella zoster, Measles, and Rubella virus antibody detection. Addition of sodium azide, adsorption of anti-human embryo lung antibodies, and 1% BSA coating vs. Standard ELISA without modifications was evaluated on Reduction of nonspecific reactions in ELISA. The addition of sodium azide to the enzyme-conjugated solution, adsorption of anti-human embryo lung antibodies, and coating wells with 1% BSA effectively eliminated nonspecific reactions in ELISA for varicella, measles, and rubella viruses.
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