Key result
ELISA variants based on blocking or binding inhibition might offer an alternative to the neutralization test for detecting poliovirus antibodies, though extensive validation is still required.
Why the study?
Does ELISA provide a reliable alternative to the neutralization test for detecting neutralizing antibodies to polioviruses?
Does ELISA provide a reliable alternative to the neutralization test for detecting neutralizing antibodies to polioviruses?
ELISA variants may serve as an alternative to neutralization tests for poliovirus antibody detection, but require large-scale validation before implementation.
ELISA variants may simplify poliovirus serology; leaves open whether they can replace neutralization testing without large-scale validation.
This review describes several enzyme-linked immunosorbent assay (ELISA) techniques proposed to replace the neutralization test for detecting neutralization-relevant antibodies to polioviruses in recipients of inactivated poliovirus vaccine and oral poliovirus vaccine, and for seroepidemiologic studies. Comparisons of results from ELISA and the neutralization test suggest that ELISA variants, based on the principle of blocking or binding inhibition that emulate the neutralization test, might offer an alternative to the neutralization test. However, to replace the neutralization test with ELISA would first require extensive studies with very large numbers of serum samples, including sera having low titers of neutralizing antibodies, in order to obtain reliable and statistically sound validation.
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Ap et al. (2005) conducted a review in Immune response to poliovirus vaccines. ELISA techniques vs. Neutralization test was evaluated on Detection of neutralization-relevant antibodies to polioviruses. ELISA variants based on blocking or binding inhibition might offer an alternative to the neutralization test for detecting poliovirus antibodies, though extensive validation is still required.
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