Key result
The poliovirus-binding inhibition test (PoBI test) showed excellent correlation (r > 0.95) with the conventional neutralization test for detecting antibodies to all three types of polioviruses.
Why the study?
Does the PoBI test correlate with the conventional neutralization test for determining antibodies to polioviruses in human serum samples?
Does the PoBI test correlate with the conventional neutralization test for determining antibodies to polioviruses in human serum samples?
Effect estimate: r > 0.95
The PoBI test is a simple, live-virus-free alternative to the conventional neutralization test for quantifying poliovirus antibodies, showing excellent correlation.
May support PoBI as simpler live-virus-free alternative for poliovirus serology; leaves open need for prospective validation before clinical use.
A poliovirus-binding inhibition test (PoBI test) was established for the quantitative determination of antibodies to polioviruses and was evaluated in comparison with the conventional neutralization test (NT). The first step of the PoBI test is an incubation of serial dilutions of test samples with inactivated poliovirus followed by the detection of free viral epitopes by a double antibody sandwich enzyme-linked immunosorbent assay with type-specific capture polyclonal antisera and type-specific neutralizing monoclonal indicator antibodies. A comparison of the PoBI test with the conventional NT for antibodies to all three types in 100 human serum samples showed excellent correlations (r > 0.95) over a wide range of antibody concentrations. The PoBI test, not necessitating live virus and tissue culture facilities, could be a simple alternative to the NT, and the principle of the assay is potentially applicable to other microbial systems.
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Edevåg et al. (1995) studied Poliovirus antibodies (n=100). Poliovirus-binding inhibition test (PoBI test) vs. Conventional neutralization test (NT) was evaluated on Correlation between PoBI test and conventional NT for antibodies to all three types of polioviruses (r > 0.95). The poliovirus-binding inhibition test (PoBI test) showed excellent correlation (r > 0.95) with the conventional neutralization test for detecting antibodies to all three types of polioviruses.
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