Key result
Binding Inhibition ELISA showed 100% sensitivity for Sabin 1, 2, and 3, and 100% specificity for Sabin 1 and 2 (97% for Sabin 3) compared to the conventional neutralization test.
Why the study?
Does Binding Inhibition ELISA accurately determine neutralization-relevant antibodies to polioviruses compared to the conventional neutralization test in OPV recipients?
Cross-Sectional (n=68)
Does Binding Inhibition ELISA accurately determine neutralization-relevant antibodies to polioviruses compared to the conventional neutralization test in OPV recipients?
The BI ELISA is a reliable alternative to the conventional neutralization test for determining neutralization-relevant antibodies to polioviruses.
Answers are grounded in this paper and cite their sources.
May support BI ELISA as alternative for poliovirus serology in OPV recipients; leaves open prospective validation before routine adoption.
This study describes an ELISA variant (Binding Inhibition ELISA, BI ELISA) for the quantitative determination of neutralization-relevant antibodies to polioviruses. The test differs from previously described analogs by utilizing polyclonal immune reagents: capture antibodies and biotin-labeled detecting antibodies. The BI ELISA was compared with the conventional neutralization test (NT) by testing live Sabin and wild-type poliovirus strains. The comparison of 68 serum samples taken from Oral Poliovirus Vaccine (OPV) recipients showed 100% specificity and sensitivity for Sabin 1 and Sabin 2, and 100% sensitivity and 97% specificity for Sabin 3. Good correlations (r = 0.7, 0.77, 0.65 for Sabin 1, 2, 3, respectively) were demonstrated between the NT and BI ELISA results. These results indicate that the BI ELISA can be used as a reliable alternative to the NT for determination of neutralization-relevant antibodies to polioviruses in vaccinees and in large-scale sero-epidemiological studies.
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Ap et al. (2005) conducted a cross-sectional in Immune response to Oral Poliovirus Vaccine (n=68). Binding Inhibition ELISA (BI ELISA) vs. Conventional neutralization test (NT) was evaluated on Sensitivity, specificity, and correlation of BI ELISA compared to NT for Sabin 1, 2, and 3 (Sensitivity 100% for Sabin 1, 2, 3; Specificity 100% for Sabin 1, 2 and 97% for Sabin 3). Binding Inhibition ELISA showed 100% sensitivity for Sabin 1, 2, and 3, and 100% specificity for Sabin 1 and 2 (97% for Sabin 3) compared to the conventional neutralization test.
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