Why the study?
Does a monoclonal antibody-based IgM capture ELISA accurately assess response to trivalent oral poliovirus vaccine compared to a neutralization assay in infants?
Does a monoclonal antibody-based IgM capture ELISA accurately assess response to trivalent oral poliovirus vaccine compared to a neutralization assay in infants?
IgM capture ELISA results may not be adequate substitutes for neutralizing antibody results when determining poliovirus vaccine response due to discordance.
IgM ELISA may overestimate OPV responses versus neutralization; leaves open its validity as a substitute in infant serosurveys.
Monoclone-based IgM capture ELISAs were developed for each of the three poliovirus serotypes and compared with a neutralization assay for detecting response to trivalent oral poliovirus vaccine among 224 infants. The IgM-based response rates were significantly higher than the neutralizing antibody-based rates: 95% versus 83% to poliovirus type 1, 99% versus 94% to poliovirus type 2, and 89% versus 59% to poliovirus type 3. IgM responses to the first vaccine dose were significantly associated between serotypes, suggesting that some of the discordance may reflect a heterotypic IgM response. When the response rates in 4 vaccine formulation groups were compared, group differences using the two assays were similar for poliovirus types 1 and 2 but not for type 3. Therefore, IgM results using these assays may not be adequate substitutes for neutralizing antibody results when determining vaccine response.
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Gary et al. (1997) studied this question.