Evaporative light scattering detection (ELSD) is an inexpensive “universal” mode of optical detection for LC. We report here on ELSD as a useful adjunct to UV detection for the RP-HPLC peptide mapping of proteins. We use peptide mapping as a “finger-printing” technique to detect post-translational modifications and other small changes to recombinant protein pharmaceuticals. UV absorption and ELSD modes were run in series for a RP-HPLC tryptic map of the small “model” protein, cytochrome c. We also report preliminary ELSD data for the RP-HPLC tryptic map of an IgG1 monoclonal antibody.
No takes yet. Share an insight, caveat, or question.
Bongers et al. (2000) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: