Reaktive copolymere des N‐vinyl‐2‐pyrrolidons zur immobilisierung von enzymen

Abstract 1‐Vinyl‐2‐pyrrolidone was copolymerized with maleic acid anhydride, with acrylic acid, and with tyrene respectively, using 1,4‐divinylbenzene as crosslinking agent. By polymeranalogous reactions isothiocyanato‐ and diazo groups were introduced into the styrene copolymers, respectively, and azido groups into the acrylic acid copolymers. These reactive, insoluble, hydrophilic carriers were used for the immobilization of enzymes by covalent binding. The copolymers, particularly their ability to immobilize papain as well as their enzymatic activity, were investigated.