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A new hierarchical porous zeolite–diatomite composite (see Figure inset) of excellent mechanical and chemical stability, after immobilization of Co2+ by an ion‐exchange process, was utilized as a packing sorbent for the separation of peptides and proteins containing histidine clusters. The Figure shows the chromatographic profile for two peptides separated using immobilized metal‐ion affinity chromatography.
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Xu et al. (2003) studied this question.
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