What question did this study set out to answer?

To elucidate the cytological mechanism responsible for mitotic arrest and somatic chromosome doubling following colchicine exposure in plant root cells.

July 9, 2010HereditasOpen Access

The Effect of Colchicine on Root Mitoses in Allium

View Full Paper

Ask AI

Bookmark

Authors

ALALBERT LEVANLankenau Institute for Medical Research

Discussion

Member takes

Implication

Laboratory study observes disrupted mitotic spindle function and chromosome accumulation in Allium root tips, highlighting the cytological mechanism behind chromosome doubling.

Key Points

To elucidate the cytological mechanism responsible for mitotic arrest and somatic chromosome doubling following colchicine exposure in plant root cells.
Exposed growing root tips (0.5–1.0 cm in length) of Allium fistulosum and Allium cepa to colchicine solutions for varying durations.
Transferred treated roots to pure water and performed tissue fixations in Navashin solution under air evacuation at designated intervals.
Sectioned embedded root tissues transversely (20 µm) and longitudinally (20–30 µm) followed by gentian violet and lichtgrün staining.
Colchicine treatment arrests mitoses by impeding the normal formation and functional spindle mechanism required for chromosome segregation.
The elevated frequency of mitotic figures represents an accumulation of blocked division stages rather than an active stimulation of cell division.
Failure of spindle-driven separation leads directly to the somatic retention of doubled chromosome sets in root cells.

Cite This Study

ALBERT LEVAN (2010) studied this question.

synapsesocial.com/papers/6aa031b003e29c6012e9ea1e https://doi.org/10.1111/j.1601-5223.1938.tb03221.x

View Full Paper

Ask AI

Bookmark

Implication

Laboratory study observes disrupted mitotic spindle function and chromosome accumulation in Allium root tips, highlighting the cytological mechanism behind chromosome doubling.

The Effect of Colchicine on Root Mitoses in Allium

Discussion

Member takes

Implication

Key Points

Cite This Study

The Effect of Colchicine on Root Mitoses in Allium

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider